This manual is intended to the undergraduate and postgraduate students in Microbiology as well as Botany and Zoology in which Microbiology is being taught as ancillary subject. This manual explains exercises in simple terms with sufficient background and principle of the experiments. Illustrations are provided along with the protocols for effective understanding the experiments. This manual deals with the experiments in basic Microbiology, Microbial Physiology Metabolism, Soil, Agricultural, Water and Medical Microbiology. It is expected that beginners and graduate students in Microbiology will be benefited from this manual.